Ceramide and Sphingosine 1-Phosphate in Liver Diseases

Woo-Jae Park, Jae-Hwi Song, Goon-Tae Kim, and Tae-Sik Park

Abstract

The liver is an important organ in the regulation of glucose and lipid metabolism. It is responsible for systemic energy homeostasis. When energy need exceeds the storage capacity in the liver, fatty acids are shunted into nonoxidative sphingolipid biosynthesis, which increases the level of cellular ceramides. Accumulation of ceramides alters substrate utilization from glucose to lipids, activates triglyceride storage, and results in the development of both insulin resistance and hepatosteatosis, increasing the likelihood of major metabolic diseases. Another sphingolipid metabolite, sphingosine 1-phosphate (S1P) is a bioactive signaling molecule that acts via S1P-specific G protein coupled receptors. It regulates many cellular and physiological events. Since an increase in plasma S1P is associated with obesity, it seems reasonable that recent studies have provided evidence that S1P is linked to lipid pathophysiology, including hepatosteatosis and fibrosis. Herein, we review recent findings on ceramides and S1P in obesity-mediated liver diseases and the therapeutic potential of these sphingolipid metabolites.

Keywords: ceramide, fibrosis, insulin resistance, obesity, sphingosine 1-phosphate, steatosis

INTRODUCTION

Sphingolipid metabolism as a sensor for FA surplus

Sphingolipid metabolism is highly coordinated by a complex network of interconnected pathways, and not simply by the availability of FA substrate. The major biosynthetic site for sphingolipids is the endoplasmic reticulum (ER), where FA and amino acids are condensed to form ceramides (Fig. 1) (Merrill, 2002). The condensation of ceramide is a major branching point in the pathway; it may be used in the synthesis of S1P or converted to other complex sphingolipids including sphingomyelins and gangliosides. The enzymes converting ceramides into complex sphingolipids are localized in the Golgi apparatus. De novo synthesis of sphingolipids is initiated from condensation of serine and palmitoyl CoA by serine palmitoyltransferase (SPT) to produce 3-ketosphinganine, followed by a series of reactions involving the enzymes 3-ketosphinganine reductase, ceramide synthase (CerS), and dihydroceramide desaturase (DES) to produce ceramide. Dihydroceramides and ceramides are transported to the Golgi apparatus and used as substrates for the enzymes that synthesize complex sphingolipids. Specifically, these include sphingomyelin by sphingomyelin synthases, gangliosides by glucosylceramide synthase, and ceramide 1-phosphate by ceramide kinase.

Figure 1

(A) Ceramide is generated by a de novo synthetic pathway and further metabolized via a salvage pathway. Once synthesized, ceramide is converted to either glucosylceramide or sphingomyelin by adding glucose ...

Another important route for ceramide metabolism is generation of S1P. In this pathway, ceramide is deacylated by ceramidases to produce sphingosine. Phosphorylation of sphingosine is catalyzed by sphingosine kinases (SphK1, 2) to generate S1P. Newly synthesized S1P is transported out of the cell by an ATP-binding cassette (ABC) transporters or by a member of the major facilitator superfamily member, spinster 2 (Spxlink) (Nishi et al., 2014; Takabe and Spiegel, 2014). After export, S1P binds to one of five S1P-specific G protein coupled receptors (S1PR1-5) and activates diverse cellular responses (Maceyka and Spiegel, 2014; Pyne and Pyne, 2010). Cellular S1P levels are tightly regulated by sphingosine levels, SphKs, and the enzymes that metabolize S1P, which include S1P lyase, two S1P-specific phosphatases (SPP1-2), and three phosphate phosphatase (LPP1-3) (Maceyka et al., 2012). S1P acts as both an extracellular and intracellular signal. These have different biological functions depending on the site of generation of the SphK involved (Schwalm et al., 2013).

Ceramide is a regulatory messenger for excess FFA

A number of researchers have suggested that ceramide synthesis can be activated by increased FFAs (Samad et al., 2006; Schilling et al., 2013); and it has been suggested that ceramide synthesis regulates uptake of FFA. Uptake and esterification of FFAs are important in initiating the production and action of ceramide and overexpression of acid ceramidase in the liver reduced not only C16- and C18-ceramides but also CD36, the FA transporter (Xia et al., 2015). Overexpression of acid ceramidase also downregulated the activity of the atypical protein kinase Cζ(PKCζ), which is activated by ceramide and stimulates lipid uptake. The finding that a ceramide analogue enhanced translocation of CD36 to the membrane via a PKCζ-dependent mechanism was interpreted as evidence that ceramide regulates FA uptake and esterification. Chaurasia et al. (2019) recently demonstrated that mice deficient in DES1 were protected from hepatic steatosis. Translocation of CD36 was stimulated by ceramide in cultured hepatocytes.

Since ceramide synthesis may reduce excess FFA, it may activate esterification of FFA into TGs. Indeed, sterol response element binding proteins (SREBPs), which are major regulators of TG and cholesterol synthesis, were activated by exogenous C16-ceramides and hepatic expression of Srebf1 and its downstream targets for FA biosynthesis including FAS and FA elongation such as Elovl6 (Jiang et al., 2015). The possible mechanism implicates atypical PKCs such as PKCλ and ζ which are ceramide effectors and inducers for hepatosteatosis and hypertriglyceridemia in mice (Chen et al., 2019; Taniguchi et al., 2006). In addition, the finding that ceramide analogues inhibited isoproterenol-stimulated phosphorylation of hormone-sensitive lipase (HSL) suggested that ceramide inhibits release of FAs from TGs (Turpin et al., 2014). Collectively, these data suggest that ceramide activates TG synthesis to relieve the FFA burden and prevents FA release from lipid droplets.

Although ceramide enhanced FA entry into cells, it inhibited the uptake of glucose (Summers et al., 1998; Wang et al., 1998). The primary effect of ceramide on glucose uptake appears to be to inhibit the insulin-responsive translocation of the GLUT4 glucose transporter to the plasma membrane, by blocking insulin-mediated phosphorylation of Akt, a serine/threonine kinase involved in insulin action, anabolic signaling, and cell survival (Hajduch et al., 2001; Summers et al., 1998; Wang et al., 1998). PKCζ activated by ceramide, phosphorylates Akt on a third inhibitory site in the enzyme’s PH domain, which reduces the kinase’s affinity for phosphoinositides and prevents its PI3 kinase-dependent activation (Powell et al., 2003). In addition, ceramide-activated protein phosphatase 2A (PP2A) enhances dephosphorylation of Akt (Zinda et al., 2001). The relative contribution of either PKCζ or PP2A pathway is dependent on cell type.

Collectively, based on these findings, we suggest that ceramide regulates lipid and glucose metabolism by modulating gene expression and signaling effectors. This mechanism is the adaptation process of the substrate oxidation to adjust to lipid-overload condition.

ROLES OF SPHINGOLIPIDS IN HEPATOSTEATOSIS AND FIBROSIS

Ceramides in hepatosteatosis

Most SM is generated by sphingomyelin synthase 1 (SMS1). Therefore, it is not surprising that SMS1-null mice exhibited moderate neonatal lethality and severe pancreatic dysfunction (Yano et al., 2011). This precludes the use of these mice in obesity experiments. Instead, mice deficient in sphingomyelin synthase (SMS 2), which are more sensitive to insulin and diet-induced obesity than WT mice (Mitsutake et al., 2011), have less PPAR-γ and its downstream target CD36, and have smaller lipid droplets. Overexpression of SMS2 in the liver had the opposite effect: it stimulated FA uptake, resulting hepatic steatosis (Li et al., 2013). Although there is a consensus that ceramide levels correlate positively with insulin resistance and development of fatty liver, SMS2 liver-specific transgenic mice had less ceramide and were more susceptible to diet-induced fatty liver formation than WT mice, while mice deficient in SMS2 had more ceramide than the WT mice and were more susceptible to diet-induced fatty liver formation. Since SMS2 is located in plasma membrane and a novel regulator of a plasma membrane microdomain, SMS2 appears to regulate FA uptake via Caveolin1 and CD36, which are also located in the microdomain (Mitsutake et al., 2011). Inhibition of glucosylceramide synthase (GCS), another enzyme that converts ceramide to glucosylceramide, also protected against HFD-induced fatty liver. These mice also had less SREBP-1c and its downstream targets, FA synthase (FAS), and stearoyl-CoA desaturase-1 (SCD-1) than mice in which GCS had not been inhibited, but PPAR-α and PPAR-γ were unaffected (Zhao et al., 2009). The exact mechanism of how GCS inhibition affects the development of fatty liver and insulin resistance is unclear, but the reduced ganglioside GM3, which plays an important role in insulin resistance (Tagami et al., 2002; Yamashita et al., 2003), could increase insulin sensitivity.

Sphingosine 1-phosphate in steatosis and obesity

The fingolimod FTY720 is a therapeutic drug for multiple sclerosis and the autoimmune disease. The drug acts as a functional antagonist of S1PR1 to induce S1PR1 degradation (Brinkmann et al., 2010). In mice fed a high-calorie diet to induce NASH, FTY720 administration reduced body and liver weight, and these effects were accompanied by decreasing hepatocyte ballooning, hepatic inflammation, and fibrosis in liver compared to mice fed a normal diet (Mauer et al., 2017). When mice fed a western diet supplemented with sweet water, the administration of FTY720 alleviated hepatosteatosis, and this was accompanied by decreasing hepatic inflammation and sphingolipid species—specifically, ceramide, dihydroceramide, S1P, and dihydro-S1P (Rohrbach et al., 2019). In contrast to mice deficient in S1PR1, the mice lacking S1PR2 rapidly developed fatty livers on a HFD (Nagahashi et al., 2015). From this evidence, it may be that the S1P receptor isotypes contribute differently to the development of fatty livers. The effects on fatty liver and insulin resistance caused by manipulation of sphingolipid levels are summarized in Table 1.

Implication of ceramides and S1P in liver fibrosis

Table 1.

The effects of sphingolipids changes on fatty liver and insulin resistance

Liver fibrosis is a chronic liver disease that results from excess production of extracellular matrix proteins (Bissell, 1998), as a result of multiple injuries, functional wound healing, or chronic liver disease. It can lead to cirrhosis or liver cancer (Kisseleva et al., 2012; Kitatani et al., 2015). During fibrosis, hepatocytes undergo apoptosis and activate hepatic stellate cells by activating Kupffer cells, which release proinflammatory cytokines (Higuchi and Gores, 2003; Pessayre et al., 2002). Activated hepatic stellate cells secrete extracellular matrix to fill the space called the Disse space, and they proliferate and replace dead hepatocytes with fibrous scar tissue; this is fibrosis (Bataller and Brenner, 2005; Schuppan and Afdhal, 2008; Shea and Tager, 2012). Thus, drugs that block or inhibit hepatic stellate cell activation may be effective in preventing fibrosis (Rippe and Brenner, 2004).

Ceramide and liver fibrosis

As ceramide accumulates, cells undergo apoptosis and trigger the fibrotic events. Fibrosis is a defense mechanism to protect the tissue from lysing cells. In mice on which fibrosis was induced with carbon tetrachloride treatment, the total amount of ceramide was increased in both plasma and liver (Ichi et al., 2007), and mice lacking acid SMase had lower ceramide levels than mice with this enzyme (Mari et al., 2008). Acid sphingomyelinase activates hepatic stellate cells, which promote fibrogenesis through promoting migration of the cells and extracellular matrix secretion (Moles et al., 2010). Ceramide regulates the expression of collagen genes, important components of the extracellular matrix, via a mechanism of “regulated intra membrane proteolysis”. Cyclic AMP response element 3 like 1 (CREB3L1) is a transcriptional factor, which regulate collagen synthesis. CREB3L1 is cleaved by two Golgi-localized protease, site-1 and site-2 proteases (S1P/S2P), leading to enter the nucleus where it binds Smad4 and then upregulates transcription of genes for assembly of collagen-containing extracellular matrix (Chen et al., 2016b). Ceramides alter the orientation of TM4SF20, a protein blocking the access of S1P/S2P to CREB3L1, and activate fibrogenic processes (Chen et al., 2016b; Denard et al., 2012). In a recent study, administration of myriocin significantly reduced ceramide levels and reduced liver inflammation and fibrosis (Jiang et al., 2019). Thus, ceramide is believed to be important in regulating apoptosis of hepatocytes. It is also a potential, major target for the treatment of NASH and fibrosis.

S1P and liver fibrosis

S1P regulates the expression of various extracellular matrices during liver fibrosis (Li et al., 2009a). S1P also activates the proliferation and migration of hepatic stellate cells in vitro and increases the expression of extracellular matrix proteins, such as α-smooth muscle actin and collagen I and III (Al Fadel et al., 2016; Friedman, 2008; Gonzalez-Fernandez et al., 2017). In liver fibrosis studies, the levels of S1P were elevated consequent to increased hepatic SphK1 expression compared to the control tissue; this was observed both in fibrous liver tissue from mouse and human patients (King et al., 2017; Li et al., 2011). The expression level of Spxlink mRNA, which encodes a transporter of S1P, was elevated in fibrotic human liver compared to normal liver, indicating increased export of S1P and its binding to specific receptors, leading to fibrosis and inflammation (Sato et al., 2016). In a recent report, workers analyzed 95 patients with end-stage liver disease and observed that patients with low concentrations of plasma S1P had a poor prognosis (Becker et al., 2017). It seems, then, that S1P has a complex role in the development of advanced liver fibrosis and cirrhosis.

S1PRs and liver fibrosis

Two S1P receptors, S1PR1 and S1PR3, are considered to be the two major S1PRs that are important in liver fibrosis. Their levels were also elevated in cholestasis-induced liver fibrosis and in human fibrotic samples, whereas the S1PR2 levels were decreased, compared to the appropriate normal controls (Li et al., 2011; Xiu et al., 2015). Antagonists of S1PR1 and S1PR3 blocked upregulation of Ang1 and alleviated fibrosis in the damaged liver, whereas the S1PR2 antagonist had no effect in angiogenesis (Yang et al., 2013). Silencing of S1PR3 diminished not only the ability of bone marrow-derived cells to migrate to the liver but also their transdifferentiation into myofibroblast-like cells (Li et al., 2009a). Recently, human embryonic lethal abnormal visual protein (HuR) was induced during liver fibrosis via S1P and increased expression of S1PR3. HuR, an mRNA binding protein, affects the vitality of bone marrow-derived cells and further stabilize their mRNA (Chang et al., 2017). On the other hand, a KO mutant of S1PR2 in animal models of liver fibrosis protects mice from the development of fibrosis (Ikeda et al., 2009), and the expression of S1PR2 was reduced in the liver analysis from the patients with liver fibrosis (Li et al., 2011). We suggest that further studies are needed to identify the roles of S1P and S1PRs in the fibrosis process induced by various cells. Major sphingolipids and the mechanism for development of fatty liver and fibrosis are summarized in Fig. 2.

Therapeutic targets in the treatment and prevention of liver fibrosis

Figure 2

During liver fibrosis, ceramide and S1P levels are elevated. Ceramide promotes PKCζ activation, which induces CD36-mediated fatty acid uptake (Xia et al., 2015) and disturbs glucose uptake (Powell et al., ...

Tracking and altering the signaling pathway of S1P may aid in the treatment of liver fibrosis. For example, the discovery of fingolimod, also known as FTY720 and a modulator of S1PR1, is promising. This drug has been approved by the U.S. Food and Drug Administration (FDA) and is the first oral drug that effectively treats recurrent multiple sclerosis. The development of inhibitors of S1P signaling and approaches that target enzymes in the sphingolipid pathway are novel fields in the search for efficient antifibrotic drugs (Dyckman, 2017; Park and Im, 2017). The major candidate drugs that mediate antifibrotic activity through the regulation of S1P or its receptors reported so far are summarized in Table 2.

Table 2.

The candidate drugs/agents of antifibrotic activity

CONCLUSION

Ceramides are the nutrient sensors that alleviate the FFA oversupply, inhibit glucose utilization, and activate deposition of TGs in the liver. Acting independently of ceramides, S1P activates cellular signaling via S1PR binding and has important roles in hepatic pathologies. While pharmacological intervention of sphingolipid biosynthesis for NAFLD is promising, more detailed understanding of the pathways is needed. It is expected that the second generation of therapeutics for liver diseases can be pursued in light of these metabolic pathways.

Article information

Mol. Cells.May 31, 2020; 43(5): 419-430.

Published online 2020-05-12. doi: 10.14348/molcells.2020.0054

Woo-Jae Park^1,*, Jae-Hwi Song², Goon-Tae Kim², and Tae-Sik Park^2,*

¹Department of Biochemistry, College of Medicine, Gachon University, Incheon 2999, Korea

²Department of Life Science, Gachon University, Seongnam 1310, Korea

^*Correspondence: ooze@gachon.ac.kr (WJP); tspark@gachon.ac.kr (TSP)

Received March 1, 2020; Accepted April 19, 2020.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

Articles from Mol. Cells are provided here courtesy of Mol. Cells

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Pathway	Chemical treated or KO mice	Insulin resistance	Fatty liver	Phenotype	Reference
De novo ceramide biosynthesis	Myriocin	Improved	Improved	Weight gain after HFD ↓ Serum ceramide ↓ Insulin signaling in liver and muscle ↑ Energy expenditure ↑ (UCP3 ↑, SOCS-3 ↓ in adipose tissue)	( Holland et al., 2007; Kurek et al., 2013; Ussher et al., 2010; Yang et al., 2009)
	DES1 KO mice	Improved	Improved	Weight gain in ob/ob mice ↓ Hepatic C16, C18, C20, C22, C24-Cer ↓ Serum C16, C18, C20, C22, C24-Cer ↓ White adipose tissue mass ↓	( Chaurasia et al., 2019)
	CerS6 KO mice	Improved	Improved	Weight gain after HFD ↓ Hepatic p-Akt, p-GSK3 ↑ Energy expenditure ↑ β-oxidation capacity in brown adipose tissue ↑ Hepatic β-oxidation ↑ PPAR-γ, CD36 ↓	( Turpin et al., 2014)
	CerS5 KO mice	Improved	Improved	Weight gain after HFD ↓	( Gosejacob et al., 2016)
	CerS2 heterozygote (+/–) mice	Aggravated	Aggravated	Serum cholesterol ↑ γImpaired lipid oxidation γImparted electron transport chain activity	( Raichur et al., 2014)
Salvage pathway	nSMase2 KO mice	No study	No study	Dwarfism phenotype Joint deformation Short statured long bones Growth hormone ↓	( Stoffel et al., 2007)
	aSMase KO mice	Improved	Improved	Hepatic stellate cells proliferation ↓ Liver fibrosis after bile duct ligation ↓ ER stress ↓	( Fucho et al., 2014)
	aSMase inhibition (amitrypsine, imipramine, desipramine)	Improved	Improved	Inflammation ↓ Serum ceramide ↓ C16-Cer, C24-Cer ↓ p-Akt, p-p70S6K ↑ p-p38, p-JNK ↓	( Fucho et al., 2014; Jin et al., 2013; Liangpunsakul et al., 2012)
	GCS inhibition (Genz-123346, Genz-112638, AMP-DNM)	Improved	Improved	FAS, SCD-1, ACC1 ↓ p-Insulin receptor-β, p-mTOR ↑ GM3 ↓	( Aerts et al., 2007; Zhao et al., 2007; 2009)
Sphingomyelin pathway	SMS1 KO mice	No study	No study	Mitochondrial dysfunction Impaired insulin secretion	( Yano et al., 2011)
	SMS2 KO mice	Improved	Improved	Weight gain after HFD ↓ PPAR-γ, CD36 ↓	( Mitsutake et al., 2011)
	SMS2 transgenic mice	No study	Aggravated	CD36 ↑	( Li et al., 2013)
Catabolic pathway	SphK1 null mice	Aggravated	Improved	Hepatic triglyceride, cholesterol ↓ Hepatic S1P ↓ PPAR-γ, CD36, UCP2, Cidea ↓ Pancreatic β-cell mass in HFD-fed ↓ Insulin production in HFD-fed ↓	( Chen et al., 2016a; Qi et al., 2013)
	SphK1 overexpression by AdSphK1	Improved	Improved	Hepatic triglyceride ↓ p-Akt, p-GSK3 ↑	( Ma et al., 2007)
	SphK2 overexpression by AdSphK2	Improved	Improved	p-Akt ↑ C16, C18, C24:1-Cer ↓ Sphingosine, sphinganine ↓ PPAR-α, CPT1, ACOX1 ↑	( Lee et al., 2015)
	FTY720 (S1PR1 antagonist)	Improved	Improved	Macrophage infiltration ↓ Ly6-C (monocyte-derived macrophages marker), CCR2 (C-C chemokine receptor type 2) ↓ α-smooth muscle actin ↓ C16-, C24:1-ceramide ↓ Fatty acid synthase ↓ S1P, dihydro-S1P ↓	( Mauer et al., 2017; Rohrbach et al., 2019)
	S1PR2 deficiency mice	No study	Aggravated	H3K9 acetylation ↓ H4K5 acetylation ↓ H2BK12 acetylation ↓	( Nagahashi et al., 2015)

Action	Drug/agent	Reference
Sphingosine kinase inhibitor	PF543 (SphK inhibitor)	( Gonzalez-Fernandez et al., 2017)
	SKI-II (SphK inhibitor, non-selective)	( Yang et al., 2013)
	N,N-dimethylsphingosine (DMS, SphK inhibitor)	( Brunati et al., 2008; Wang et al., 2017b; Xiu et al., 2015)
S1P receptor agonist/antagonist	FTY720 (S1PR1 and S1PR3 agonist)	( Brunati et al., 2008; King et al., 2017; Kong et al., 2014)
	VPC23019 (S1PR1 and S1PR3 antagonist)	( Brunati et al., 2008; Yang et al., 2012; 2013)
	SEW2871 (S1PR1 agonist)	( Ding et al., 2016)
	W146 (S1PR1 antagonist)	( King et al., 2017; Liu et al., 2011; Yang et al., 2012; 2013)
	JTE-013 (S1PR2 antagonist)	( Kageyama et al., 2012; Wang et al., 2017a; Xu et al., 2016; Yang et al., 2015)
	Suramin (S1PR3 antagonist)	( Li et al., 2009a; 2009b)
	KRP203 (FTY720 analog)	( Kaneko et al., 2006; Khattar et al., 2013)
	CAY-10444 (S1PR3 antagonist)	( Yang et al., 2015)
	VPC24191 (S1PR1 and S1PR3 antagonist)	( Al Fadel et al., 2016)
Other inhibitor	Pertussis toxin (PTX; G-protein-coupled receptor signaling inhibitor)	( Brunati et al., 2008; Gonzalez-Fernandez et al., 2017; Yang et al., 2015)
Other inhibitor	Melatonin (melatonin receptors agonist)