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Mol. Cells 2009; 27(2): 263-268

Published online February 28, 2009

https://doi.org/10.1007/s10059-009-0032-0

© The Korean Society for Molecular and Cellular Biology

Transcriptomic Analysis of Rat Brain Tissue Following Gamma Knife Surgery: Early and Distinct Bilateral Effects in the Un-Irradiated Striatum

Misato Hirano, Junko Shibato, Randeep Rakwal, Nobuo Kouyama, Yoko Katayama, Motohiro Hayashi, Yoshinori Masuo

Received: November 5, 2008; Revised: November 24, 2008; Accepted: November 25, 2008

Abstract

Gamma knife surgery (GKS) is used for the treatment of various human brain disorders. However, the biological effects of gamma ray irradiation on both the target area, and the surrounding tissues are not well studied. The effects of gamma ray exposure to both targeted and un-targeted regions were therefore evaluated by monitoring gene expression changes in the unilateral irradiated (60 Gy) and contralateral un-irradiated striata in the rat. Striata of irradiated and control brains were dissected 16 hours post-irradiation for analysis using a whole genome 44K DNA oligo microarray approach. The results revealed 230 induced and 144 repressed genes in the irradiated striatum and 432 induced and 239 repressed genes in the un-irradiated striatum. Out of these altered genes 39 of the induced and 16 of the reduced genes were common to both irradiated and un-irradiated tissue. Results of semi-quantitative, confirmatory RT-PCR and western blot analyses suggested that ?-irradiation caused cellular damage, including oxidative stress, in the striata of both hemispheres of the brains of treated animals.

Keywords brain, gamma irradiation, gene expression, rat model

Article

Communication

Mol. Cells 2009; 27(2): 263-268

Published online February 28, 2009 https://doi.org/10.1007/s10059-009-0032-0

Copyright © The Korean Society for Molecular and Cellular Biology.

Transcriptomic Analysis of Rat Brain Tissue Following Gamma Knife Surgery: Early and Distinct Bilateral Effects in the Un-Irradiated Striatum

Misato Hirano, Junko Shibato, Randeep Rakwal, Nobuo Kouyama, Yoko Katayama, Motohiro Hayashi, Yoshinori Masuo

Received: November 5, 2008; Revised: November 24, 2008; Accepted: November 25, 2008

Abstract

Gamma knife surgery (GKS) is used for the treatment of various human brain disorders. However, the biological effects of gamma ray irradiation on both the target area, and the surrounding tissues are not well studied. The effects of gamma ray exposure to both targeted and un-targeted regions were therefore evaluated by monitoring gene expression changes in the unilateral irradiated (60 Gy) and contralateral un-irradiated striata in the rat. Striata of irradiated and control brains were dissected 16 hours post-irradiation for analysis using a whole genome 44K DNA oligo microarray approach. The results revealed 230 induced and 144 repressed genes in the irradiated striatum and 432 induced and 239 repressed genes in the un-irradiated striatum. Out of these altered genes 39 of the induced and 16 of the reduced genes were common to both irradiated and un-irradiated tissue. Results of semi-quantitative, confirmatory RT-PCR and western blot analyses suggested that ?-irradiation caused cellular damage, including oxidative stress, in the striata of both hemispheres of the brains of treated animals.

Keywords: brain, gamma irradiation, gene expression, rat model

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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