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" /> Wenzhu Jiang, Joohyun Lee, Yong-Mei Jin, Yongli Qiao, Rihua Piao, Sun Mi Jang, Mi-Ok Woo, Soon-Wook Kwon, Xianhu Liu, Hong-Yu Pan, Xinglin Du*, and Hee-Jong Koh*

" /> Wenzhu Jiang, Joohyun Lee, Yong-Mei Jin, Yongli Qiao, Rihua Piao, Sun Mi Jang, Mi-Ok Woo, Soon-Wook Kwon, Xianhu Liu, Hong-Yu Pan, Xinglin Du*, and Hee-Jong Koh*

. Mol. Cells 2011;31:385-92. https://doi.org/10.1007/s10059-011-0049-z">
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Mol. Cells 2011; 31(4): 385-392

Published online April 30, 2011

https://doi.org/10.1007/s10059-011-0049-z

© The Korean Society for Molecular and Cellular Biology

Identification of QTLs for Seed Germination Capability after Various Storage Periods Using Two RIL Populations in Rice

Wenzhu Jiang1, Joohyun Lee2, Yong-Mei Jin3, Yongli Qiao2, Rihua Piao2, Sun Mi Jang2, Mi-Ok Woo2, Soon-Wook Kwon4, Xianhu Liu5, Hong-Yu Pan1, Xinglin Du1,*, and Hee-Jong Koh2,*

1College of Plant Science, Jilin University, Changchun 130062, China, 2Department of Plant Science, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea, 3School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Korea, 4Department of Agricultural Sciences, Korea National Open University, Seoul 110-791, Korea, 5Department of Agricultural Sciences, Yanbian University, Yanji 133000, China

Correspondence to : *Correspondence: heejkoh@snu.ac.kr (HJK); duxinglin2004@163.com (XD)

Received: January 5, 2011; Revised: January 24, 2011; Accepted: January 24, 2011

Abstract

Seed germination capability of rice is one of the impor-tant traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chro-mosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive x additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.

Keywords near isogenic line, quantitative trait loci, rice, RIL, seed germination capability

Article

Research Article

Mol. Cells 2011; 31(4): 385-392

Published online April 30, 2011 https://doi.org/10.1007/s10059-011-0049-z

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of QTLs for Seed Germination Capability after Various Storage Periods Using Two RIL Populations in Rice

Wenzhu Jiang1, Joohyun Lee2, Yong-Mei Jin3, Yongli Qiao2, Rihua Piao2, Sun Mi Jang2, Mi-Ok Woo2, Soon-Wook Kwon4, Xianhu Liu5, Hong-Yu Pan1, Xinglin Du1,*, and Hee-Jong Koh2,*

1College of Plant Science, Jilin University, Changchun 130062, China, 2Department of Plant Science, Research Institute of Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Korea, 3School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Korea, 4Department of Agricultural Sciences, Korea National Open University, Seoul 110-791, Korea, 5Department of Agricultural Sciences, Yanbian University, Yanji 133000, China

Correspondence to:*Correspondence: heejkoh@snu.ac.kr (HJK); duxinglin2004@163.com (XD)

Received: January 5, 2011; Revised: January 24, 2011; Accepted: January 24, 2011

Abstract

Seed germination capability of rice is one of the impor-tant traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chro-mosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive x additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.

Keywords: near isogenic line, quantitative trait loci, rice, RIL, seed germination capability

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
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Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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