Lifespan assays are either performed with solid or liquid media. Solid culture systems using agar-based media are a major method for lifespan assays. Liquid culture systems using S-media are widely used for testing the effects of chemicals on lifespan. Synchronized worms at a specific developmental stage are transferred for manual or automatic counting, and results are then analyzed using various statistical tools.|@|~(^,^)~|@|C. elegans survival assays that measure resistance against various abiotic stresses, such as oxidative stress, heat, cold, osmotic stress, hypoxia, hyperoxia, ultraviolet radiation, endoplasmic reticulum stress, and heavy metal stress, contribute to the investigation of C. elegans responses to internal or external stresses. Shown are various stress-inducing agents with typical assay conditions.|@|~(^,^)~|@|Shown are representative pathogenic bacteria, fungi, and parasites used for C. elegans pathogen resistance assays. Specific assays using P. aeruginosa (PA14), the most popular model pathogen for C. elegans, are depicted briefly; slow-killing assays that use small-lawn or big-lawn assays and fast-killing assays are shown.

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